Composite
Part:BBa_K2957035:Design
Designed by: Melody Wu Group: iGEM19_MIT (2019-10-19)
TRE-tight, mKate
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30
Illegal XbaI site found at 1051 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30
Illegal XbaI site found at 1051 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 322
Illegal XbaI site found at 30
Illegal XbaI site found at 1051
Illegal AgeI site found at 1057 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 784
Illegal BsaI.rc site found at 973
Design Notes
We needed to consider which cell type this plasmid would go into and the transfection efficiency was often dependent on promoter in the genetic circuit/construct.
Source
This part was from mentor Shiva Razavi, who gave us the part when we were interested in potentially pursuing the HL60 cell to HEK cell communication synNotch system as a proof of concept of chemotaxis.