Composite

Part:BBa_K2957035:Design

Designed by: Melody Wu   Group: iGEM19_MIT   (2019-10-19)


TRE-tight, mKate


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal XbaI site found at 1051
    Illegal AgeI site found at 1057
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 784
    Illegal BsaI.rc site found at 973


Design Notes

We needed to consider which cell type this plasmid would go into and the transfection efficiency was often dependent on promoter in the genetic circuit/construct.


Source

This part was from mentor Shiva Razavi, who gave us the part when we were interested in potentially pursuing the HL60 cell to HEK cell communication synNotch system as a proof of concept of chemotaxis.


References